6 CapitalPress.com July 29, 2016 Quest for the best apple Kate Evans leads most advanced apple breeding program in world By DAN WHEAT Capital Press WENATCHEE, Wash. — When Kate Evans was a little girl in Shefield, England, she enjoyed pulling apart plants in her parents’ garden. She was seeing how they were made. She was looking for lower and leaf buds. “My parents were ine with it. We had lots of lowers. There were enough to spare,” she says with a smile. Forty years later, she’s de- veloped and manages what is arguably the most advanced apple breeding program in the world. It’s the irst to use DNA testing for fruit quality, in other words aimed at the best irmness, crispness, juic- iness, lavor, sweetness and storability of apples. Some European apple breeding programs were irst in applying DNA testing to disease resistance, she said. Evans came to the Wash- ington State University Tree Fruit Research and Extension Center in Wenatchee sev- en years ago as an associate professor of horticulture and pome fruit (fruit produced by lowering plants) breeder and head of the center’s apple breeding program. She suc- ceeded Bruce Barritt who re- tired and had started the pro- gram 14 years earlier. Evans had her doctorate in plant molecular biology and had spent 16 years leading apple and pear breeding pro- grams for East Malling Re- search, in England. When she arrived in Wenatchee, the center’s apple breeding program was just heading toward DNA testing. “It was in its absolute in- fancy, so a lot of the work I’ve focused on is how to use that technology to develop the lo- gistics of the application be- cause it has to be 100 percent accurate regarding the trace- ability of the data to the indi- vidual seedlings,” she said. “When you use the data, you remove or throw away the seedling. So it’s termi- nal selection. If you make a mistake and throw away the wrong one, you’ve wasted ev- erything,” she said. DNA testing is done at seedling selection stage and prior to that in selecting the most efficient parent combinations to produce Kate Evans Age: 49 Born and raised: Shefield, England Family: Husband, Peter Smytheman, entomologist and research intern WSUT- FREC. Children: Thomas, 16; Chloe, 1 Education: Bachelor’s degree in genetics and plant- biology, Leeds University; doctorate in plant molecular biology, Durham University Occupation: Associate professor of horticulture and pome fruit breeder, WSU Tree Fruit Research and Extension Center, Wenatchee, Wash. desired characteristics. Previously, parents were chosen for their general fruit and tree characteristics, but with DNA genetic markers parents can be better selected AI.OW16-4/#6 Dan Wheat/Capital Press Kate Evans, Washington State University apple breeder, looks at fruit from breeding selections at the WSU Tree Fruit Research and Extension Center in Wenatchee. The apples came out of cold storage and were brought to room temperature for a week before being measured for irmness, crispness, lavor and juiciness. to reduce the number of poor progeny. A genetic marker is a gene or short sequence of DNA that’s a good indicator of a speciic trait or character- istic. “You can have markers for single traits, markers for mul- tiple traits and several mark- ers for the same trait. Not often is a trait controlled by a single gene,” she said. “We know acidity is pre- dominately controlled by two major genes, but for other more complicated characteristics the DNA tests only explain a portion of the overall characteristic,” Ev- ans said. A lot of research is going into identifying more DNA markers. But it is slow work. The USDA-funded Ros- BREED project led by genet- icists Amy Lezzoni, professor of plant breeding at Michigan State University and Cameron Peace, associate professor of horticulture at WSU in Pull- man, is making progress. Quality and storability are the overriding targets of the WSU apple breeding pro- gram. Producers want fruit that pleases consumers and stores well for year-long sales. More speciically, Ev- ans uses DNA testing to aim for desired levels of lavor or sweetness versus tartness (sugar versus acidity), irm- ness, texture and ripening. It takes about 18 years from crossing parents for hy- brid seed to reach commercial release of a new variety. “The DNA technology is more focused on eficiency than speed, but in ive years we may be at a point of hav- ing suficient DNA markers for important characteristics that we will be able to take out one of the selection phases and save three to four years,” Evans said. Three new varieties were released from the program in recent years, all from Bar- ritt’s breeding. WA 2 didn’t gain traction in the industry because there was no com- mercial name and companies were leery of marketing a va- riety that could end up with multiple names. WA 5 had some long-term storability issues. WA 38, released over the past several years, was given the name Cosmic Crisp by WSU in 2014. Proprietary Va- riety Management, a Yakima company that specializes in variety management, is assist- ing WSU with the apple. Nurseries budded trees this past fall and there may be 500,000 trees, instead of 300,000, divvied out to grow- ers by lottery for planting in the spring of 2017, Evans said. This story originally ap- peared Nov. 27, 2015.